Morphological and biochemical characterization of bacterial species of Bacillus, Lysinibacillus and Brevibacillus

The objective of this work was to characterize reference bacteria strains, belonging the genus Bacillus and species correlated genera, by simplified morphological biochemical methods. characterization is based on aspects colonies, as well cytomorphology species, optical scanning microscopy. For characterization, sensitivity test antimicrobials disk-diffusion performed. Moreover, strains were characterized extracting intracellular proteins. Characteristics such shape, color, consistency in addition type spore production protein crystals determinants for these species. antibiogram revealed high resistance β-lactam group antibiotics, cereus s.l group. In subtilis s.l. there susceptibility antibiograms, mainly B. subtilis. profile provided specific patterns some bands 130 e 65 kDa thuringiensis, 140 Lysinibacillus sphaericus, 115 Brevibacillus laterosporus. Our results showed that characterizations, a simple identification, with easy interpretation, low cost.